Writing your Manuscript

You’ve done your research – now it’s time to share it with the world! 

A manuscript is a written report of a research project. It describes what researchers studied, how they performed research, what they found, and what their data and results mean. Manuscripts are one of the main ways scientists share their research.

Please follow these steps to prepare your manuscript for submission to eiRxiv. To make things easier, we’ve also provided a downloadable submission checklist (link to PDF) and manuscript template (link to PDF).  

  1. Read these guidelines, the submission checklist , and the manuscript template. 
  2. Write your manuscript on the manuscript template
  3. Check your manuscript against these guidelines, the submission checklist, and the template. Have you followed all of the instructions and met all of the requirements? If you have, you’re ready to submit. If not, please make any necessary revisions first. 

 

As a reminder, manuscripts that aren’t on our template or don’t meet our formatting guidelines will not pass prereview.

Writing Your Manuscript

Manuscript Format & Content

References

Figures & Tables

Common Mistakes

Manuscript Format & Content

Manuscript Formatting

We have strict requirements regarding your manuscript font, layout, and structure. Please use our manuscript template to ensure you meet all of these requirements: 

  • 1.5x Line Spacing
  • Font: Arial Black (Title: 16pt Bold; Headings: 11pt Bold; All other text: 10pt, unbolded)
  • 1-inch margins on all sides
  • No columns for text
  • 10 page limit counted from the start of the Introduction to the end of the Materials/Methods
  • 8 Table + Figure maximum
  • Do not use specially formatted headings or subheadings – they should all be bolded 11pt font

**The best way to ensure you keep this formatting is to type directly into our template. Don’t copy/paste into it!**

Once you have finalized your manuscript, you will need to convert it to a PDF in order to submit. For more information, see Exporting a Word document to a PDF. Or, if you’re using our Word Document template within Google Docs, How to Download a Google Doc as a PDF.

Manuscript Content

Your manuscript should include the following sections, in this order. Please see the submission checklist (link to PDF) and manuscript template (link to PDF) for full details on what’s expected. You can also read other eiRxiv and JEI manuscripts for examples.

 

📄Title

 Your manuscript should begin with a title, your names and schools. This includes:

  • Project Title – A clear and descriptive title for your project 
  • Author Names – List student authors (First authors) and adult authors (Last authors; adults who supervised your project), their institutions, and any individuals who did equal work. See our guidance on Listing Authors for more details on how to format this.

A short paragraph (150-250 words) that summarizes and highlights the importance of your research. You can include:

  • A brief statement of why your research question is important
  • What research questions you asked
  • How you did your research
  • Your main results and what they mean

Background information to help readers understand your research, including:

  • What scientists already know about your research topic (from books, articles, or websites)
  • The specific research question you try to answer
  • Why you chose this research project and why your research question matters
  • Your hypothesis

Describe and show what you found. Please keep in mind:

  • Report your data (results) using numbers, tables, or graphs
  • Keep your results clear and organized
  • Focus on reporting your data, not interpreting it. You will explain and interpret your data in the ‘Discussion’
  • Please see our Statistics Guide (link PDF) for full guidance on how to collect, analyze, and present data

Explain what your results mean. You can also interpret your results from different angles:

  • Interpret  your results and explain what they mean. 
  • Compare your results to what other studies have found
  • Mention any limitations of your study (factors you couldn’t control or sources of potential errors).
  • When you write your discussion, please be careful not to make your results sound stronger than they are. For example, instead of saying ‘This proves…,’ you can say ‘Our results showed….’ or’ These findings support…’’
  • Make sure you’re not overstating your conclusions, or making statements beyond what your experiments show. You can propose reasons or extensions for what you observed, but unless this was directly shown by your experiments, you should make it clear that these are just speculations.  
  • Suggest ways your research could be used to guide future studies, or what additional research would help answer more questions. One way to think about what comes next is to ask yourself: What other experiments could help confirm or expand my results? Did my results raise new questions?

 

Note: The discussion should make up a large portion of your manuscript, because it is where you explain your results and what they mean, link them to previous research, and provide ideas for the future.

This section is like a “recipe” for your science, where you clearly describe how you did your experiments so others could repeat and learn from them. Your materials and methods should include: 

  • What materials you used (e.g. equipment, surveys, and samples)
  • How you set up your experiments
  • How you collected and analysed data

 

Unlike a recipe, please do not write a separate list of materials like an “ingredients” section, and do not write your methods as a bulleted or numbered list. Instead, write in full sentences and paragraphs and describe your materials as part of your method. For example, “We measured bean growth by placing seeds in small pots filled with potting soil. Each pot was watered with 10 ml of tap water every day and kept under either red, blue, or green light for two weeks.”

For more on references, see this section.

For more on figures, see this section.

For more on acknowledgements, see this section.

For more on appendices, see this section.

References

Choosing and Giving Credit to Your Sources

As a scientist, choosing and referencing reliable sources shows everyone: 

  • You have given proper credit to any other authors or scientists for their ideas
  • You are getting your supporting information from credible scientific sources

 

When any scientist shares their work, any fact they state in their manuscript must be properly cited. In fact, not citing your sources correctly is considered plagiarism and can have serious negative consequences for a scientist’s career. 

At eiRxiv and JEI, manuscripts that have not properly cited their sources will be sent back to the authors and – in severe cases – may be rejected. Please review JEI’s Academic Honesty page for more information on when you need to cite sources, how you should cite them, and how to avoid unintentional plagiarism.

Choosing good, trustworthy sources of scientific information provides a solid foundation for your research. If your research is based on faulty or biased information, your conclusions may not be accurate, other scientists may not trust your results, or your work could mislead readers. 

Good sources should be: 

  • Scientific: written by scientists or other experts in the field, and accepted or verified by other scientists/experts
  • Unbiased: written to test a hypothesis or show data, not to prove a point, sell a product/service, or support an agenda
  • Factual: based on reliable data that has been properly analyzed and interpreted, not based on opinions
  • Current: reflecting the most up-to-date understanding of the field and incorporating any new discoveries or changes

 

Scientific journal articles are the best place to find your information, but textbooks, science magazines / news articles, preprints, and encyclopedias can also be useful. We don’t recommend citing Wikipedia, blogs, social media, etc. as sources of scientific information, though they may be able to point you toward other, more scientific resources as you investigate. 

The following sites can help you access scientific journal articles: 

PubMed: research articles on a variety of subjects, mostly biomedical

PubMed Central: like PubMed, but excludes research articles that have paywalls

Google Scholar: research articles on all scientific topics

Directory of Open Access Journals: additional subject-specific journals that don’t require a subscription.

You should also check with your school or local library to see what services and search engines they provide for scientific resources (e.g. EBSCO, Elsevier, etc.).

After planning, it’s time to do the experiment! You collect data carefully, making sure to accurately document everything you observe. To make sure your data are reliable, you need to repeat your experiment several times. A good rule of thumb is at least 3 trials for each condition (called replicates). More trials are even better if you have time and resources.

Once you have collected your data, you can organize your data into tables or graphs so it’s easier to see patterns or differences. You can also use statistics (link to guide) to determine whether your results happened by chance, or because you’re observing a consistent pattern.

Once you analyze your data, you can think about what your results mean. You can ask yourself questions like: Did the results match what I thought would happen? Did anything surprise me? If your experiment didn’t work as planned, that’s okay! That’s normal in science. What matters most is what you learned from your project. You can suggest ways to improve the experiment or come up with new questions to explore. That’s how professional scientists make discoveries: by learning from both expected and unexpected data. Every experiment is a chance to discover something new, even if the results aren’t what you expected.

Decide who you want to share your research with, and how you want to communicate your idea with them. Sharing your science can take many forms: conversations, presentations, social media, posters, scientific journal articles, preprints, and more!

Figures and Tables

How to show your data

Figures (graphs or pictures) and tables (grids of numbers) help your readers understand your results by providing visual representations of your research. You can have up to a total of 8 Figures or Tables in your eiRxiv manuscript. 

  • Tables can help you organize and compare numerical values or group information. When you use a table, please make sure:
    • Row and column headings are easy to read and understand
    • Numbers are clearly lined up and well spaced so they’re easy to compare. 
  • Figures (diagrams, images, and graphs) can help you show a relationship, process, pattern, layout, difference, or other type of visual image. 
    • Graphs: Every axis needs a clear, understandable label and units. Some graphs may also need a legend. If you collected the same measurement more than once, you should include error bars to let readers know how much the data varies. 
    • Images: Make sure your images are clear, high resolution, and focused. If size matters for your images, please use scale bars.
    • Diagrams: Ensure the diagrams are easy to follow and use simple, clear labels.

 

Captions for Figures and Tables

Rather than putting titles on figures and tables, eiRxiv asks authors to instead use captions: a short explanation that goes under your figure. A caption tells your reader what your figure is about so they can understand your results without reading your entire manuscript. Your caption can include:

    • Title (must be included) – what the figure is about
    • What is pictured (must be included) – What your figure or table shows, and where the data came from
    • Statistics – If you calculated values like means or statistical significance
    • Replicates – If you repeated your experiment, you can mention it in your caption
  • Any additional information necessary for the reader to understand your figure/table


For full details on how to format your figures and tables, add captions, and reference your figures/tables in your text, please see JEI’s Figure/Table Formatting Guide. We recommend reading this before you create your figures or tables.

  • The figures and tables you use in your manuscript should be your own, original creation. If you see a figure or table from someone else that you feel is critical to include in your own manuscript, you must: 
  1. Get written approval from the eiRxiv editorial team. They will work with you to determine if there is a way to omit the original figure/table. 
  2. Get and submit written permission from the author and the original publication in order to avoid copyright infringement.
  • If you used software like Biorender or Canva that requires a license to publish generated figures or use templates, you must submit a copy of the license with your manuscript. Note that the free version of Biorender does not allow for the publication of generated figures.
  • Please do not use generative AI to create your figures. Generative AI may pull from copyrighted images, causing your image to unknowingly infringe on other creators’ copyright. In addition, generative AI has been known to create scientifically inaccurate or misleading images.

Acknowledgements (Optional)

This is where you can thank people who helped you with your research project or manuscript.

Who to thank here:

  • Friends, classmates, or family who gave you feedback on your project or manuscript
  • People who helped you with supplies or let you use their equipment.
  • Anyone who gave you advice but is not an author on the manuscript
  • If you got money or supplies from a group, school, or program for your project, you can thank them here too.

 

Who Not to thank here:

  • If you have listed your teacher and mentors as authors on your manuscript, being an author is the way you show their contribution and your appreciation. You do not need to acknowledge your authors again here.
  • People who did not contribute to your project or manuscript

 

*Please make sure you get written permission from anyone you name in your acknowledgements to include them before you submit your manuscript. The easiest way to do this is to email them and ask, and then save that email. That way you have a permanent record that they agreed to be in your acknowledgements.

Appendix (optional)

eiRxiv allows appendices (an extra section in your manuscript for supplemental information) on a case-by-case basis. 

We do accept the following for appendices: 

  • Code or programming scripts: These should be placed in the appendix or shared through a public repository like GitHub (Links to private storage like Google Drive are not accepted)
  • Survey questions: If your study used a survey, you can include the full list of questions in the appendix.

 

An appendix is NOT a place to simply add more information to your manuscript, or a way to get around the 10-page limit. If your appendix doesn’t fit the two situations above, you can still submit it, but you must also include a statement explaining to the editors why the material belongs in the appendix instead of in the main text of the manuscript.

Common Mistakes

We understand this is a very new way of writing for most student authors, with many detailed requirements. 

Before you begin writing, we recommend reading JEI’s Common Mistakes guide – it identifies many of the common structural, grammatical, formatting, and content mistakes student authors make. Because eiRxiv preprints do not go through a formal peer review and revision process, you should check for all of these mistakes before you submit your manuscript.